A Bacterial Cytotoxin Identifies the RhoA Exchange Factor Net1 as a Key Effector in the Response to DNA Damage (original) (raw)
Figure 3
Net1 knock down prevents actin stress fiber formation upon intoxication.
A) HeLa cells non-transfected, or transfected with control or Net1 specific shRNA or siRNA, were left untreated or treated with CDT (2 μg ml−1) for 24h. The actin cytoskeleton was visualized by TRITC-phalloidin staining (red). B) Quantification of cells with actin stress fibers (mean±SD of 5 independent experiments: three performed with shRNA and two performed with siRNA). One hundred and fifty cells were counted for each experiment. Cells carrying more than 5 stress fibers were scored as positive. C) The fluorescence intensity per each cell was quantified using the ImageJ software. Data are presented as ratio between the mean fluorescence intensity and the cell area. iRNA is defined as in Figure 2B.