A Bacterial Cytotoxin Identifies the RhoA Exchange Factor Net1 as a Key Effector in the Response to DNA Damage (original) (raw)
Figure 9
ROCK inhibitors do not prevent DNA damage-induced p38 MAPK phosphorylation.
A) HeLa cells, were left untreated or pre-treated with the specific ROCK inhibitors H-1152 or Y27632 (10 μM) in complete medium for 1h prior to irradiation (20 Gy), and further incubated in the presence or absence of the inhibitor for 12h. The actin cytoskeleton was visualized by FITC-phalloidin staining (green). B) HeLa cells, pre-treated with the specific ROCK inhibitors H-1152 or Y27632 (10 μM) in complete medium for 1h, were left untreated or exposed to IR (20Gy), and further incubated in the presence or absence of the inhibitors for the indicated periods of time. p38 MAPK phosphorylation was assessed as in Figure 5A.