Laboratory Evolution of Fast-Folding Green Fluorescent Protein Using Secretory Pathway Quality Control (original) (raw)
Figure 3
In vitro characterization of GFP folding.
(a) Kinetic refolding measured as fraction folded (Ff) over time, (b) equilibrium unfolding measured as Ff versus GdnHCl molarity and (c) kinetic unfolding measured as Ff over time for the different GFP variants as indicated. Curve fits were added as a visual aid. All data is the average of three replicate experiments where the standard error for all data was <10%.