Dynamics of Dynamin during Clathrin Mediated Endocytosis in PC12 Cells (original) (raw)
Figure 5
Different phases of dynamin intensity decrease at sites of clathrin-mediated endocytosis in PC12 cells.
PC12 cells co-transfected with clathrin-dsRed and dynamin1-EGFP (A) or dynamin2-EGFP (B) were imaged by live-cell TIR-FM. Decreases in dynamin fluorescence were aligned so that the peak was set to time 0, and clathrin and Dynamin fluorescence at time 0 were normalized to 1. (B and C) demonstrate the decreases in clathrin and dynamin1 or dynamin2, at the sites of 30 or 28 disappearing clathrin spots, respectively. In each case, the decrease in clathrin intensity fits a single exponential, while both dynamin1 and dynamin2 fit double exponentials (all r2 values >0.98).