HyperISGylation of Old World Monkey ISG15 in Human Cells (original) (raw)
Figure 2
OWmISG15s conjugate more efficiently to target proteins.
ISGylation pattern of FLAG-tagged Human, Chimpanzee, Rhesus Monkey, African Green Monkey and Mouse ISG15 in (A) human HekT cells, (B) monkey COS cells and (C) mouse N38 cells. Cells were only transfected with expression vectors for the indicated ISG15 orthologues, without extra stimulation. Upper panels: Western blot analysis on total cell lysates showing the total ISGylation pattern after ectopic expression of the indicated ISG15 orthologues as visualized by their FLAG-tag (anti-FLAG Ab). Lower panels: actin loading control (anti-actin Ab).