HyperISGylation of Old World Monkey ISG15 in Human Cells (original) (raw)
Figure 6
Residue 89 in ISG15 is situated in the predicted contact area with its Activating enzyme.
(A) ISG15 (red ribbons) overlaid to Nedd8 (green ribbons) bound to its Activating enzyme AppBp1-Uba3 (grey ribbons). Residue 89 (dark blue) of ISG15, makes contact with residues in the two β-strands (shown in brown) of the Activating enzyme that precedes its C-terminal domain. K193 in Uba3 (orange) is located very close to N89, and corresponds to W568 in human UbE1L. The other residues of ISG15 with an important effect on total ISGylation, situated at positions 133 (yellow) and 31–33 (cyan blue), do not make contact with UbE1L and are likely to be involved in another feature of the ISGylation process. (B) HuUbE1L was aligned with HuUbE1 and RhmUbE1L (Ensembl peptide sequence ENSMMUP00000027609). The region 563–569 in HuUbE1L shows two substitutions (S565L and W568R, red coloured) in the corresponding region of RhmUbE1L, which better resembles the corresponding region in HuUbE1 (K607).