Colorectal Cancer Stem Cells Are Enriched in Xenogeneic Tumors Following Chemotherapy (original) (raw)
Figure 1
CoCSC phenotype cells preferentially survive CPA chemotherapy.
A) Phenotypic profile of UM-C4 colorectal tumors for ESA, CD44 and CD166, following exclusion of mLin− cells. B) Formalin fixed, paraffin embedded tumor sections from CPA- or vehicle-treated control mice stained with Hematoxylin & Eosin (H&E), for proliferating Ki-67+, or for TUNEL+ dead cells. Black bar = 100 µm. C) Following randomization to normalize treatment groups at 400 mm3 at day 0, twice weekly administration of vehicle (green boxes) or 38 mg/kg CPA (red circles) commenced and tumors were measured periodically. D) Phenotypic analysis of individual tumors, displaying the percentage of human tumor cells with the ESA+CD44+ phenotype as a function of tumor size. E) Representative overlay histogram displaying CD166 surface expression on human ESA+CD44+ tumor cells from vehicle- or CPA-treated animals. The black line represents isotype control staining of ESA+CD44+ tumor cells.