Colorectal Cancer Stem Cells Are Enriched in Xenogeneic Tumors Following Chemotherapy (original) (raw)
Figure 4
CoCSC with high ALDH1 activity are more prevalent following CPA therapy.
A) Ratio of ALDH+ tumor cells among the human ESA+CD44+ population in tumors from vehicle-treated control or CPA-treated mice. Data reflects the paired Mean of 5 independent experiments using 3 different xenogeneic colorectal tumor lines (UM-C4, UM-C6 & OMP-C8) and n≥5 mice per experiment. B) Taqman qRT-PCR data for the denoted genes using TG and NTG populations isolated from (V) vehicle- or CPA-treated UM-C4 tumors. Data represents Mean±SEM (n≥2). C) Immunofluorescence or immunoperoxidase staining of frozen or formalin-fixed, paraffin embedded tumors for ALDH1 or c-Myc, respectively, from vehicle- and CPA-treated mice. Black bar = 100 µm.