PINK1 Is Necessary for Long Term Survival and Mitochondrial Function in Human Dopaminergic Neurons (original) (raw)
Figure 2
Long term viability is reduced in PINK1 deficient neurons.
A) Graph showing an increase in mean CI over time of human PINK1 kd neurons compared to controls. Values shown are means±s.e.m of 3 control and 4 kd cultures, each measured in triplicate. B) Graph showing an increase in mean CI over time of mouse embryonic cortical neuronal cultures taken from wild type and PINK1 knockout mice. Values shown are means±s.e.m of wild type (PINK1+/+, n = 2), and homozygote (PINK1−/−, n = 3) cultures each measured in triplicate. C,D,E) Comparison of individual cell parameters assayed by the Cytotoxicity algorithm for aged human neurons (dd 52) with PINK1 kd compared to controls, including mean nuclear size, mean nuclear intensity, plasma membrane permeability and lysosomal mass/pH. Values shown are means±s.e.m of 3 control and 4 kd cultures, each measured in triplicate. F) Histogram showing percentage apoptotic neuronal nuclei in aged control and PINK1 kd human neurons under basal conditions. Values shown are mean percentages of neuronal nuclei that are pyknotic from 10 fields of view, ±sem from 3 independent cultures.