Intestinal Bacteria Condition Dendritic Cells to Promote IgA Production (original) (raw)
Figure 1
PP-DC promote IgA production by B cells.
(A) The dot plot (left panel) shows IgM and IgD expression by MACS sorted splenic IgD+ B cells. Gates depict putative B2 and B1 cell populations. The histogram plot (right panel) shows CD5 expression by splenic IgD+ B cells (shaded area) or peritoneal B cells (solid line) gated for CD19 expression. (B–D) Splenic IgD+ B cells from naïve C57BL6 mice were cultured alone (open bars) or together with CD11c+ DC isolated from the PP (black bars) or PLN (grey bars). Anti-CD40 mAb (2 µg/ml) was added to all cultures in the absence or presence of additional LPS (1 µg/ml) as indicated. At the end of 7 days of culture, supernatants were collected and (B) IgA, (C) IgG1 and (D) IgM antibody concentrations determined by standard ELISA assay. Cultures were performed in triplicate and the mean±S.E.M. are shown. B.D. depicts those samples where the antibody concentration was below the detection limit of the ELISA assay. No IgA was detected in control cultures containing DC alone. The data shown are from one experiment and are representative of at least three independent experiments.