Intestinal Bacteria Condition Dendritic Cells to Promote IgA Production (original) (raw)
Figure 4
PP-DC promoted IgA production requires BCMA and TACI signaling in the absence of CD40- or LPS-mediated signals.
Naïve splenic IgD+ B cells from C57BL/6 (plain bars) or TACIxBCMAo/o mice (striped bars) were cultured together with CD11c+ DC from the PP (black and white bars) or PLN (grey bars). (A–C) Anti-CD40 mAb (2 µg/ml) was added to all cultures in the absence or presence of additional LPS (1 µg/ml) as indicated. (D–F) Cultures were performed in the absence of anti-CD40 mAb but contained LPS (1 µg/ml) where indicated. At the end of 7 days of culture, supernatants were collected and (A&D) IgA, (B&E) IgG1 and (C&F) IgM antibody concentrations determined by standard ELISA assay. Cultures were performed in triplicate and the mean±S.E.M. are shown. B.D. depicts those samples where the antibody concentration was below the detection limit of the ELISA assay. No IgA was detected in cultures containing DC alone. The data shown are from one experiment and are representative of two (D–F) and three (A–C) independent experiments.