Intestinal Bacteria Condition Dendritic Cells to Promote IgA Production (original) (raw)
Figure 5
Intestinal commensal bacteria are partially responsible for the ability of PP-DC to promote IgA production.
Naïve splenic IgD+ B cells from C57BL/6 mice were cultured alone (open bars) or together with CD11c+ DC from the PP (black bars) or PLN (grey bars). CD11c+ DC were isolated from mice maintained under SPF or GF conditions as indicated. Anti-CD40 mAb (5 µg/ml) was added to all cultures in the absence or presence of additional LPS (1 µg/ml) as indicated. At the end of 7 days of culture, supernatants were collected and (A) IgA, (B) IgG1 and (C) IgM antibody concentrations determined by standard ELISA assay. Cultures were performed in triplicate and the mean±S.E.M. are shown. B.D. depicts those samples where the antibody concentration was below the detection limit of the ELISA assay. No IgA was detected in control cultures containing DC alone. The data shown are from one experiment and are representative of three independent experiments.