Tankyrase 1 and Tankyrase 2 Are Essential but Redundant for Mouse Embryonic Development (original) (raw)
Figure 2
Expression of TANK1 and TANK1a in WT and TANK1−/− mouse tissues.
All RNA samples were serially diluted as indicated for PCR amplification and analysis. Actin cDNA was used as an RT-PCR loading control in all experiments. (A) RT-PCR analysis for TANK1 mRNA expression in various tissues of WT and TANK1−/− (KO) mice as indicated. 5′-TANK1 RT-PCR products represent upstream cDNA of TANK1. (B) RT-PCR analysis for TANK1 mRNA expression in WT and TANK1−/− (KO) testis as indicated. 5′-TANK1 and 3′-TANK1 RT-PCR products represent upstream and downstream cDNAs of TANK1; and TANK1a RT-PCR products are specific for TANK1a. (C) RT-PCR analysis for TANK1a mRNA expression in various tissues of WT mice as indicated. TANK1a RT-PCR products represent cDNA of TANK1a. (D, E) Western blot analysis was used to determine tankyrase 1 and 1a expression in thymus, testis and spleen of WT and TANK1−/− (KO) mice with 762 (anti-SAM, D) and 376 (anti-HPS, E) antibodies. TANK1 indicates tankyrase 1 protein, TANK1x indicates a possible degraded tankyrase 1 protein, and TANK1a indicates tankyrase 1a protein.