AIP4/Itch Regulates Notch Receptor Degradation in the Absence of Ligand (original) (raw)
Figure 7
Notch does not interact directly with Itch/AIP4.
The Notch IC+CT, IC-CT, numb and DTX proteins were in vitro-translated in the presence of 35S met, and their ability to be retained onto a GST-AIP4 fusion protein or control GST adsorbed to glutathione-agarose beads was analyzed by SDS-PAGE analysis. The input lanes (7, 8, 15, 16, 23, 24, 25) show the different in vitro-translated products prior to incubation with the beads. Two concentrations of in vitro translated DTX (0.5 or 3 µl, indicated by + and + respectively) were tested. White lines indicate that intervening lanes have been spliced out.