ATP Release from Vascular Endothelia Occurs Across Cx43 Hemichannels and Is Attenuated during Hypoxia (original) (raw)

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Figure 2

Molecular mechanisms of endothelial-dependent ATP release.

A–D, Confluent HMEC-1 monolayers were washed and exposed to Verapamil (10 µmol) or Dipyridamole (1 µmol) over 20 min, 18-alpha-glycyrrhetinic acid (18αGA, 20 µmol) or Anandamide (40 µmol) over 10 min. ATP content within the supernatant was measured by a luminometric ATP detection assay and compared with untreated control cells (n = 6).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0002801.g002