Poly A- Transcripts Expressed in HeLa Cells (original) (raw)
Figure 3
Experimental verification of novel 3′ ESTs.
(A). 3′ end verification for each subtype of 3′ EST. 3′ ESTs from poly A-, poly A+, and bimorphic subtypes were selected for the confirmation. Known poly A+ transcripts were used as positive control. Random priming- generated cDNA and oligo dT-generated cDNA were used as the templates. R: cDNA generated by random priming; T: cDNA generated by oligo dT priming. See Table S7 for primer information. (B). Verification of 3′ ESTs mapped to intronic microRNA precursors. RT-PCR was used to verify the 3′ ESTs that map to intronic microRNA precursors. Amplified products were cloned and sequenced. See Table S4C for primer information. (C). northern blot verification of poly A- 3′ EST. Two poly A- 3′ ESTs were used as the probes (Table S7) and RNAs from five human cell lines were used for the detection.