In Vitro Analysis of Integrated Global High-Resolution DNA Methylation Profiling with Genomic Imbalance and Gene Expression in Osteosarcoma (original) (raw)
Figure 3
Gene-specific real-time PCR validation of the Me-DIP-chip data.
Me-DIP-chip detected genes in Table 1 were subject to real-time PCR quantitative analysis of enrichment. The y-axis represents fold enrichment values generated by calculating the ration of U2OS or MG63 IP (Ct)/IN(Ct) over osteoblast IP(Ct)/IN(Ct). Each real-time PCR reaction was performed in triplicate and average values were used for enrichment calculation.