The Histone H3 Lysine 27-Specific Demethylase Jmjd3 Is Required for Neural Commitment (original) (raw)
Figure 1
Expression profiling of JmjC-domain containing proteins identifies Jmjd3 as a protein specifically upregulated at the outset of neural commitment.
(a) ES cell-derived neural stem (NS) cells constitute a homogeneous population of neural precursors. They show uniform expression of neural precursor markers nestin and Sox2 and virtually no immunoreactivity for neuronal (β-tubulin III) or astrocyte (GFAP) antigens. (b) Expression of JmjC-genes at day 8 and 26 of ES cell to NS cell differentiation. mRNA levels were quantified by real-time RT-PCR. For each gene the transcript level in wild-type ES cells was set as 100%. The bars represent the means±S.D. of triplicates normalized to TBP. (c) Protein expression levels of Jmjd3 during the ES cell to NS cell transition. Levels of Jmjd3 peak at days 4 and 8 and are downregulated in established NS cell cultures. Protein extract from cells overexpressing Jmjd3 was used to localize the Jmjd3 band (WB-western blot control). Vinculin served as a loading control.