The Histone H3 Lysine 27-Specific Demethylase Jmjd3 Is Required for Neural Commitment (original) (raw)
Figure 2
Jmjd3 is required for neural commitment.
(a) Protein levels of Jmjd3 in RNAi knock-down ES cell clones assessed by Western Blot. Vinculin served as a loading control. (b) Immunostaining for nestin (upper panel, second row) and pan-cytokeratins (lower panel, second row) and phase-contrast images (first rows of both panels) of wild type (w.t.) and Jmjd3 knock-down (Jmjd3-kd1 and Jmjd3-kd2) cells at day 7 of monolayer differentiation. Sixteen contiguous squares, providing representative coverage of the culture dishes, were imaged at 10X magnification with the same exposure time for wild type and Jmjd3 knock-down cells. Mean grey values were calculated with the ImageJ software. (c) Close-ups showing illustrative examples of the differentiating cultures shown in (b) Immunostaining for nestin (left panel, second row) and pan-cytokeratins (right panel, second row) and phase-contrast images (first rows of both panels) of wild type (w.t.) and Jmjd3 knock-down (Jmjd3-kd1 and Jmjd3-kd2) cells at day 7 of monolayer differentiation imaged with the same exposure time for wild type and Jmjd3 knock-down cells.