A Predominant Role for Parenchymal c-Jun Amino Terminal Kinase (JNK) in the Regulation of Systemic Insulin Sensitivity (original) (raw)
Figure 2
Effects of irradiation dosage on engraftment and body weight.
Mice that differ in CD45 cell surface markers were irradiated with 9, 10, and 12 Gy and transplanted with bone marrow (1×106 bone marrow cells) and the rate of chimerism in blood cells were determined by sorting with the specific antibodies for CD45.1 and CD45.2. In an additional control experiment, FACS analysis was performed without BMT using blood cells from C57BL/6J (CD45.2+) and B6.SJL PtprcaPep3b/BoyJ (CD45.1+) mice (A). FACS analysis using blood cells from CD45.1→CD45.2 BMT in mice that were irradiated with 9 Gy (B), 10 Gy (C), or 12 Gy (D). Body weight change in the three groups of mice (irradiated with 9, 10, and 12 Gy) following BMT on a regular diet (E). Percentage of reconstitution was calculated and displayed in each graph.