Signal Regulatory Protein α Negatively Regulates β2 Integrin-Mediated Monocyte Adhesion, Transendothelial Migration and Phagocytosis (original) (raw)
Figure 5
TNFα and MCP-1–induced cell spreading and actin polymerization in THP-1 cells.
SIRPα– or mock–transfected THP-1 cells were stimulated with TNFα or MCP-1 for 30 minutes, fixed, and labeled with rhodamine-conjugated phalloidin to visualize actin filaments. As a negative control, mock–transfected THP-1 cells were pretreated with cytochalasin D (Mock+Cyt. D). Bar = 20 µm.