Cancer Genes Hypermethylated in Human Embryonic Stem Cells (original) (raw)
Figure 3
Loss of promoter DNA methylation during in vitro differentiation of hESCs.
(A) Left-hand images, Shef-1 stem cell line (upper) and the same cells after neural differentiation (middle) and spontaneous differentiation to fibroblast-like cells (lower). The right-hand panels show the relative mRNA levels of pluripotency (NANOG, OCT4), neuroectodermal (PAX6, NEUROD1), and mesodermal (COL1A1, FN1) markers before and after Shef-1 differentiation. (B) Number of sequences hypomethylated during Shef-1 neural (red circle) and spontaneous (blue circle) differentiation, and their overlap with Class B-I and Class B-II genes (black circles). (C) Bisulfite genomic sequencing of multiple clones of the DLC1 promoter in Shef-1 stem cell line (upper) and the same cells after neural differentiation (middle) and spontaneous differentiation to fibroblast-like cells (lower). The color code is as for Figure 2. (D) Relationship between DLC1 promoter hypermethylation and expression during differentiation of Shef-1 cells. The upper panel shows the relative methylation signal obtained with the methylation arrays and the lower panel the expression levels of DLC1 mRNA relative to GAPDH.