Pre-miRNA Loop Nucleotides Control the Distinct Activities of mir-181a-1 and mir-181c in Early T Cell Development (original) (raw)
Figure 5
Mature miRNAs produced from the mir-181a-1/c mutants have the same 5′ ends.
The 5′ ends of mature miR-181a (A) and miR-181c (B) produced from mir-181a-1/c domain swapping mutant genes, and the 5′ ends of mature miR-181a (C) produced from mir-181a-1 loop mutant genes were determined by primer extension analyses. Synthetic miR-181a or miR-181c oligo nucleotides in single nucleotide increments (15 nt–22/23-nt) were radio-labeled and used as size ladders. The upper band represents the major cDNA product of miR-181a (23-nt) or miR-181c (22-nt), and the lower band represents radio-labeled probes for the mature miRNAs.