Pre-miRNA Loop Nucleotides Control the Distinct Activities of mir-181a-1 and mir-181c in Early T Cell Development (original) (raw)
Figure 6
The effects of mir-181a-1/c mutants on mature miRNA expression in BOSC 23 and DP cells.
(A, B) The copy numbers of mature miR-181a (A) and miR-181c (B) expressed in BOSC 23 cells transfected with the same amounts of vectors expressing different mir-181a-1/c mutants determined by quantitative Northern blot analyses (Fig. S8 for standard curves and quantification, and Table S4 for statistics). (C, D) The copy numbers of mature miR-181a (C) and miR-181c (D) expressed in DP thymocytes transduced with viral vectors expressing mir-181a-1/c mutants determined by miRNA qPCR analyses (Tables S5 and S6 for statistics). (E) The copy numbers of mature miR-181a expressed in BOSC 23 cells transfected with the same amounts of viral vectors expressing various mir-181a-1 loop mutants determined by quantitative Northern blot analyses (Fig. S9 for standard curves, and Table S7 for statistics). (F). The copy numbers of mature miR-181a expressed in DP thymocytes transduced with viral vectors expressing various mir-181a-1 loop mutants, determined by miRNA qPCR analyses (Table S8 for statistics). Statistical significance was determined by an unpaired two-tailed student's t test (compared to the control vector, red *, p<0.05). Representative blots of four or more independent quantitative Northern blot analyses are shown (A, B, E).