c-Myc Is Required for Maintenance of Glioma Cancer Stem Cells (original) (raw)
Figure 6
Depletion of c-Myc attenuates neurosphere formation by glioma cancer stem cells.
(A) T3359 CD133+ cells were infected with lentivirus and selected as described. Cells were then plated at 100 or 10 cells per well in 24-well plates in the presence of 1 µg/ml puromycin. Eight wells were plated for each group. The numbers of neurospheres in each well were determined in seven days. Spheres that contained more than 20 cells were scored. (B) Percentage of wells without neurospheres formed was quantified. #, p<0.001 with one-way ANOVA comparison of the control groups to the corresponding c-Myc knockdown groups. (C) Representative photographs of neurospheres formed by cells expressing non-targeting shRNA or c-Myc specific shRNA (bars = 50 µm). (D, E) Similar results were demonstrated in T4302 CD133+ cells.