Prdm Proto-Oncogene Transcription Factor Family Expression and Interaction with the Notch-Hes Pathway in Mouse Neurogenesis (original) (raw)
Figure 7
Prdm16 is upregulated in response to Hes1 overexpression.
pEF-EGFP and pEF-Hes1 together (A–D) or a control consisting of pEF-EGFP alone (E–H) were electroporated into telencephalic neural progenitors at E13.5. Eighteen hours later, the brains were harvested, sectioned coronally, and then ISH was carried out to detect GFP (A, C, E, G), Prdm16 (B, F), and Ngn2 (D, H). Hes1 electroporation into the telencephalon caused upregulation of Prdm16 and a concomitant downregulation of Ngn2. Scale bar in A, 500 µm.