At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1 (original) (raw)
Figure 8
Tissue distribution of the Irm transcripts in the mouse embryo revealed by in situ hybridization.
(A). On embryonic day 9.5 (E9.5), Irm expression is detected in the forebrain area (FB), first and second branchial arches (BA), in developing somites (Sm), and in the caudal neural tube (NT). A dispersed staining is observed in the extraembryonic tissues, particularly in the yolk sac (YS). (B). By E10.5 the brain expression domain shifts more in the midbrain area (MB) where the neuroepithelial lining shows the Irm staining. Similar to E9.5, expression persists both in the branchial arches and in the somites. (C). In E12 mouse conceptus, Irm transcripts could be also visualized in the forming limb cartilage (LmC), still clearly detectable in the telencephalon (TE) and caudal somites. The yolk sac (YS) and the maternal side of placenta (MP) are positive for Irm by in situ staining as well. The expression along the midline in the caudal neural tube (NT) is most likely associated with the floor plate area (D). In the head, prominent Irm transcript accumulation is observed in the trigeminal area (Tg), ear pinna (EP), and the developing whiskers hair follicle (WF, see panel E). (F). Hybridization with the sense Irm probe under the same conditions resulted in no specific signal.