Post-Translational Inhibition of IP-10 Secretion in IEC by Probiotic Bacteria: Impact on Chronic Inflammation (original) (raw)
Figure 8
Intestinal segment specific effects of VSL#3 on experimental inflammation correlate with IP-10 expression in IEC.
IL-10−/− mice were fed VSL#3 (1,3×109 cfu/mouse/day) (n = 14) or placebo (n = 12) analogous to wt mice (n = 9) for 21 weeks. Mice were sacrificed at 24 weeks of age and histopathological scoring was performed by blindly assessing the inflammation between 0 (not inflamed) and 12 (highly inflamed). Figure A and B show the median histopathologic score of the cecum and colon of IL-10−/− mice in the study. (C) Cecal and colonic IEC were isolated together and proteins (50 µg) as well as mRNA of all mice in a group were analyzed for IP-10 expression by Western blot or RT-PCR (D) Immunohistochemical staining of IP-10 in cecal tissue sections was performed. The upper row shows representative pictures (three mice per group were analysed) of stained tissue sections (IP-10 (green), DAPI (blue)) and the lower row shows representative false colour confocal images of the stained tissue sections ((IP-10) green, DAPI (red)). Arrows indicate regions that show elevated IP-10 expression.