Ser276 Phosphorylation of NF-kB p65 by MSK1 Controls SCF Expression in Inflammation (original) (raw)
Figure 3
Effect of MSK1-mediated p65 Ser276 phosphorylation on IL-1β-induced p65-CBP interaction.
A. Human lung fibroblasts in culture were transfected with 1 µg of pHis-p65 plasmid (WT p65 subunit with a N-terminal His-Tag), and pre-incubated for 1 h with a combination of SB202190 (SB; 3.5 µM) and PD98059 (PD; 20 µM), or with H89 (10 µM) and treated with IL-1β (20 U/ml). B. Human lung fibroblasts in culture were transfected with pHis-p65 or pHis-p65 S276C plasmids (1 µM), and treated with IL-1β (20 U/ml). His-p65 and co-precipitating CBP were revealed by immunoblotting using anti-p65 and anti-CBP antibodies. IP: immunoprecipitation; IB: immunoblot. Results are representative of three independent experiments performed in fibroblasts from three different donors.