TNF-alpha Is Required for the Attraction of Mesenchymal Precursors to White Adipose Tissue in Ob/ob Mice (original) (raw)
Figure 3
Analysis of the migration properties of MPs.
A. In vitro transmigration of MPs after 6 h in the presence or absence of different conditions: mock, SN (600 µl of adypocytes supernatants), recombinant murine TNFα (Rec TNF, 50 ng/ml) (*p<0.03), anti-TNF mAbs (600 µl of adypocytes supernatants in the presence of 10 µg/ml antibodies) (+p<0.05), and MCP1 (20 ng/ml). B. Images taken from the Transwells filters after 6 h under the different conditions. C. In vivo migration into the different tissues of MP population after 6 h of C57 or ob/ob iv injection, in the presence of anti-TNF-alpha mAbs treatment (*p<0.05). Note the reduction of MPs in the adipose tissue of ob/ob mice (red arrow). D. Images taken from the adipose tissue after 6 h. Note the disappearance of injected MPs around the tissue.