Differences in the Tumor Microenvironment between African-American and European-American Breast Cancer Patients (original) (raw)
Figure 3
Autocrine mobility factor receptor (AMFR) and HLA-D protein expression in breast tumors.
(A) Shown is the protein expression of AMFR in extracts of 6 tumors from African-American patients and 6 tumors from European-American patients. β-actin and calnexin are loading controls. Like AMFR, calnexin is located in the endoplasmic reticulum. (B) Quantification of AMFR expression. Shown is the relative expression of AMFR protein after normalization to β-actin. AMFR expression is significantly higher in tumors from African-American patients than in tumors from European-American patients (P = 0.038; Wilcoxon rank sum test). (C) Differential HLA-D protein expression in ER-negative breast tumors. Shown is the expression of HLA-D in extracts of 6 tumors from African-American patients and 6 tumors from European-American patients. (D) Quantification of HLA-D expression. Shown is the relative expression of HLA-D protein after normalization to β-actin. The expression is significantly higher in tumors from African-American patients than in tumors from European-American patients (P = 0.016; Wilcoxon rank sum test).