Rapid Regulatory T-Cell Response Prevents Cytokine Storm in CD28 Superagonist Treated Mice (original) (raw)
Figure 1
T-cell responses to mouse CD28SA D665 in vivo.
(A) Cell numbers in pooled lymph nodes and spleen, d3. (B) T-cell subsets in lymph nodes. (C) Frequency (%) of CD25+Foxp3+ Treg cells among CD4 lymph node T-cells (left) and their absolute numbers in pooled lymph nodes (right). (D) Cell division of conventional and regulatory CD4 T-cells, d3 after stimulation with 100 µg CD28SA. 107 purified CFSE-labeled CD4 T-cells were transferred i.v. on day −1. Average numbers of cell divisions for Foxp3-positive and –negative subsets (histogram inserts), and percentages of recovered subsets (dot plot inserts) are indicated. (E) Kinetics of absolute Treg cell numbers in lymph nodes (left), and of percentage among CD4 cells (right). *** indicates p<0.0001 as compared to untreated mice (d0).