Rapid Regulatory T-Cell Response Prevents Cytokine Storm in CD28 Superagonist Treated Mice (original) (raw)
Figure 2
Suppressive activity of CD28SA stimulated Treg cells.
(A) In vivo stimulation with CD28SA increases potency of Treg cells in vitro. CFSE-stimulated indicator CD4 T-cells were cocultured with purified Treg cells from naïve or d -3 CD28SA stimulated mice at the ratios indicated, and stimulated as given in Methods. Cell division was measured on d3, and average numbers of divisions are given as inserts. (B) CD28SA stimulation suppresses EAE by activating the Treg compartment. Left: prophylactic, middle: therapeutic treatment. Right: Treg depletion abolishes therapeutic effect. 50 µg of CD28SA were applied and EAE was read as given in Methods.