Rapid Regulatory T-Cell Response Prevents Cytokine Storm in CD28 Superagonist Treated Mice (original) (raw)
Figure 5
CD28SA mediated Treg activation is compatible with anti-phlogistic corticosteroid therapy.
(A) Dexamethasone (Dex) effectively suppresses cytokine release induced by the superantigen SEB in the presence or absence of CD28SA stimulation. Sera of mice treated as indicated (see also Methods) were analyzed 1 hr (closed bars) or 4 hrs (stripped bars) after stimulation with SEB and/or CD28SA. (B) Increase in Treg cells is unaffected by Dex treatment. Analysis on day 3 after single stimulation with SEB and/or CD28SA, and daily treatment with 5 mg/kg Dex. (C) CD28SA-induced enhancement of Treg activity is resistant to Dex treatment. Treg cells isolated from groups shown in (B) were cocultered with CFSE-labeled indicator cells as described in Methods. Cell division was measured on d3, and average numbers are given as inserts.