CFTR Is a Negative Regulator of NFκB Mediated Innate Immune Response (original) (raw)
Figure 1
CFTR downregulates IL-8 and NFκB reporter activities.
HEK293 cells were transiently transfected with wt-CFTR and IL-8 (A) or NFκB (B) reporter constructs together with a renila luciferase internal control plasmid (n = 3). The cells were induced with 1 ng/ml IL1-β overnight. Data are normalized to the renila luciferase internal control and expressed as mean±SD. A. IL-8 promoter driven luciferase expression. IL1-β stimulates IL-8 promoter activity and wt-CFTR blunts both control and IL1-1β driven IL-8 (*p<0.05). B. NFκB driven luciferase expression. IL-1β stimulates NFκB promoter activity and wt-CFTR blunts both control and IL-1-β driven NFκB (*p<0.05).