CFTR Is a Negative Regulator of NFκB Mediated Innate Immune Response (original) (raw)
Figure 4
The CFTR knock out mice show significant decline in body weight after LPS treatment.
The FABP-CFTR gut corrected CFTR knock-outs (CFKO) and wt- mice (n = 12) were injected i.p. with 15 mg/kg body weight of P. aeruginosa LPS, and body weights were recorded daily for 6 days. The CFKO mice show significant decline in body weight as compared to wild type mice (p<0.001; day 3–6). Wild type mice (blue) recover from weight loss by day 6 while CFKO mice (red) don't show significant recovery. The 25% of CFKO mice died during the course of experiment due to LPS induced chronic inflammation (green). The data suggest that CFTR is critical for LPS induced immune response and recovery from LPS induced inflammation. The data are shown as body weight in grams of each mouse in wt- and CFKO- group from day 0 to 6. The non-linear logarithmic regression was used to calculate the trend lines indicating the changes in body weight in wild type (blue) and CFKO (red) mice after LPS exposure.