MicroRNA Expression in Human Omental and Subcutaneous Adipose Tissue (original) (raw)
Figure 1
Relationships between miRNA (miR-132 and miR-198) expression and morphology of adipose tissue.
(A) Typical example of omental adipose tissue morphology demonstrating lower macrophage infiltration in a patient with high miRNA-132 expression (miR-132 high) compared to a patient with low miRNA-132 expression (miR-132 low). Macrophage infiltration was assessed by immunostaining for CD68 as described in the methods. Upper slides, magnification 20×, lower slides, magnification 10× (B) Mean omental adipocyte diameter in a patient with high miRNA-198 expression (miR-198 high) compared to a patient with low miRNA-198 expression (miR-198 low). Aliquots of adipocytes were fixed with osmic acid and counted in triplicate using Multisizer III (Beckman-Coulter, Hamburg, Germany).