Glutamine Deprivation Induces Abortive S-Phase Rescued by Deoxyribonucleotides in K-Ras Transformed Fibroblasts (original) (raw)
Figure 2
Expression of proteins involved in the G1 to S transition in normal and transformed cells.
For the protein expression analysis normal cells (A) and transformed cells (C) grown in media containing 4 mM glutamine, 1 mM glutamine and 0.5 mM glutamine were collected at appropriate time points and 40 µg of proteins from the total cellular extract were subjected to SDS-PAGE followed by Western blotting with appropriate antibodies. One of at least three independent experiments is shown. (B1–B4 and D1–D4) Relative quantitative analysis of protein levels after Western blot film acquisition by scanner and densitometry analysis using Image J program. The densitometry values obtained for each protein were normalized by using the values of the corresponding vinculin and were plotted considering the densitometry value obtained for the sample 24 hours/4 mM glutamine as equal 1. Results are the mean of at least triplicate determinations with SD indicated.