Neurogenin2 Directs Granule Neuroblast Production and Amplification while NeuroD1 Specifies Neuronal Fate during Hippocampal Neurogenesis (original) (raw)
Figure 3
Malformation of the hippocampal DG structure in Ngn2 null mutant animals.
(A1–A3) Hematoxylin/Eosin staining performed on coronal sections from two-weeks old WT, Ngn2+/GFP and Ngn2GFP/GFP mutant animals, shows that the loss of Ngn2 results in the formation of a smaller hippocampus and primitive and undeveloped DG. (B1–B4) The malformation of the DG in Ngn2GFP/GFP mutant animals can already be observed two days after birth, throughout the hippocampal structure, along the rostro-caudal axis (see also supplementary figure 2). The inlets of rectangular panels in bottom right corners show a schematic drawing of the shape of the DG for Ngn2+/GFP and Ngn2GFP/GFP mutant animals; black arrowheads point to the undeveloped and missing dorsal blade and ventral blade of the DG, respectively, in Ngn2GFP/GFP mutant animal. Scale bars: 200 µm (A1–B4).