Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping (original) (raw)
Figure 1
Development of real-time metabolotyping (RT-MT) to analyze bacterial metabolic dynamics.
In vivo 1H-NMR (right) and two-dimensional 1H, 13C-HSQC with 13C labeled substrate technique (2D-13C-HSQC) (left) were continuously conducted during bacterial growth in an NMR tube at 37°C, and the profiling data were processed and analyzed by several statistical methods, such as Z-score analysis, principal components analysis (PCA), and time series of statistical total correlation spectroscopy (STOCSY). From the results of statistical analyses of In vivo 1H-NMR profiling data, we extracted meaningful data related to time-varying information. Furthermore, from the results of in vivo 2D-13C-HSQC profiling data, we revealed the metabolic kinetics of specific metabolic reactions.