Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping (original) (raw)
Figure 3
Principal components analysis (A) and loading plot analysis (B) of three strains of B. fibrisolvens.
MDT-10 (red), MDT-5 (blue), and A38 (purple) are shown. Data under conditions of LA addition (open squares), LNA addition (close squares), or no addition (close circles) were calculated. Data are representative of three independent experiments. (A) Hourly 1H-NMR profiling data of three strains of B. fibrisolvens incubated with or without LA or LNA were analyzed. Contributions of PC1 and PC2 were 77.0% and 11.6%, respectively. All 0 h samples were assembled at the left side of Figure 3A, indicating that metabolic conditions before bacterial growth were similar. However, after 10 h, the samples were located in the right side of Figure 3A, suggesting that PC1 means bacterial growth and PC2 means metabolic differences among B. fibrisolvens strains. (B) Blue peaks and words indicate PC1 contribution and those in red indicate PC2 contribution. The 4.2–5.2 ppm region was omitted to eliminate the effects of imperfect water suppression.