Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping (original) (raw)
Figure 5
LNA metabolic dynamics of B. fibrisolvens MDT-10 analyzed by time-dependent 2D-13C-HSQC RT-MT.
The NMR tube was anaerobically inoculated with B. fibrisolvens MDT-10 and U-13C18 LNA (2.5 mM) was added to follow LNA hydrogenation reactions in in vivo 2D-13C-HSQC RT-MT. A: Typical 2D HSQC spectra (sequential growth at 0, 2, 3, and 4 hours) are shown. 2D HSQC spectra were observed every 8 minutes. Arrows in upper panel indicate C-H structures corresponding to the signals. Signal intensities were calculated based on their standard curves. Arrows pointing to lower panel (B) indicate 2D HSQC spectra measured at the indicated time point. B: LNA metabolic dynamics of B. fibrisolvens MDT-10 calculated from time-dependent 2D-13C-HSQC RT-MT. LNA (black circles), CLNA (blue triangles), _t_11, _c_15–18:2 (pink diamonds), VA (green squares), and bacterial growth (open red squares) are shown. Mean values of triplicate experiments are shown.