Evaluation and Characterization of Bacterial Metabolic Dynamics with a Novel Profiling Technique, Real-Time Metabolotyping (original) (raw)
Figure 6
Metabolic dynamics of LNA and _t_11, _c_15–18:2 in three strains of B. fibrisolvens analyzed by time-dependent 2D-13C-HSQC RT-MT.
A: When the initial OD was approximately 0.5, U-13C18 LNA or _t_11, _c_15–18:2 (2.5 mM) was added to cultures to avoid growth inhibition. LNA (black circles), CLNA (blue triangles), _t_11, _c_15–18:2 (pink diamonds), _t_11, _c_13–18:2 (orange circles), VA (green squares), and bacterial growth (open red squares) are shown. A38 did not metabolize _t_11, _c_15–18:2 (data not shown). Mean values of triplicate experiments are shown. B: LNA hydrogenation pathway of three strains of B. fibrisolvens deduced from the results of this study. Chemical structures and location of double bonds of LNA and its metabolites are shown. Arrows indicate enzymes responsible for each reaction. Light blue, red, and dark blue arrows represent LNA isomerase, CLA-R, and _t_11, _c_15–18:2 isomerase, respectively.