Heparanase Regulates Levels of Syndecan-1 in the Nucleus (original) (raw)
Figure 3
Heparanase enzymatic activity is required for reduction of syndecan-1 levels in the nucleus.
Nuclear and non-nuclear fractions were prepared from CAG cells expressing high levels of wild-type heparanase (HPSE-high) or heparanase mutated at either amino acid 343 (M343) or amino acid 225 (M225) which renders them enzymatically inactive. All cells were prepared using pIRES2 vectors for transfections. Fractions were analyzed for syndecan-1 levels by A) western blotting and B) ELISA. Grey bars = non-nuclear fraction; Black bars = nuclear fraction. Error bars represent standard error of the mean. *, P<0.01 vs. nuclear syndecan-1 in HPSE high cells.