Targeting and Function of the Mitochondrial Fission Factor GDAP1 Are Dependent on Its Tail-Anchor (original) (raw)
Figure 3
TA-associated critical basic residues for GDAP1 localization and function.
(A) Schema of GDAP1 and aa sequence of C-terminal part of wt GDAP1 (GDAP1) and mutants (bold letters: altered aa sequence). (B) Confocal immunofluorescence microscopy of transfected COS-7 cells expressing mtEGFP as control (a–d) wt GDAP1 (e–h) and mutant proteins (i–x). Co-stainings: MitoTracker (mitochondria), PDI (ER). Bars, 10 µm. (C) Quantification of GDAP1 and mutants colocalized with MitoTracker. (D) Quantification of mitochondrial morphology.