Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis (original) (raw)
Figure 1
Simplified schematic representation of Clostridium thermocellum cellulosomal architecture.
CipA is the backbone scaffoldin protein containing 9-Type I cohesins and can accommodate up to 9-Type I dockerin bearing catalytic units. CipA also contains a Type II dockerin for cell-surface attachment via anchor proteins and a Cellulose Binding Domain for attachment to the growth substrate. C. thermocellum genome encodes five proteins with Type II cohesins, four with S-layer homology domain (SdbA, OlpB, Orf2p and Cthe0735) for cell-surface anchoring of Type II dockerin bearing CipA and one without the SLH domain (Cthe0736). Also shown is the only subunit containing a Type II dockerin (Cthe1806) – (Figure adapted with permission from Carlos Fontes, CIISA, Portugal).