Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis (original) (raw)
Figure 2
Clostridium thermocellum growth profile on various substrates, based upon protein levels.
Pellet protein (top panel) and supernatant protein (bottom panel) profiles of Clostridium thermocellum during growth on various biomass carbohydrates, as estimated by BCA and Bradford protein assays, respectively. Data represented is the average of two biological replicate fermentations of C. thermocellum in minimal medium containing 5 g/L total of the following carbon substrates: Cellulose with 15N labeled nitrogen source, Cellulose with 14N labeled nitrogen source, Cellulose-Xylan (3∶2 weight ratio), Cellulose-Pectin-Xylan (3∶1∶1), and Cellulose-Pectin (3∶2). Data is not shown for Z-Trim®, pretreated switchgrass and cellobiose fermentations.