Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis (original) (raw)
Figure 3
Clostridium thermocellum cellulosomal protein profile during growth on various substrates, analyzed by protein gel electrophoresis.
SDS-PAGE (4–20% Tris-HEPES-Glycine gel, coommassie stain) separation of Clostridium thermocellum cellulosomal fractions isolated using the affinity digestion method from cell-free broth of duplicate fermentations during growth on pretreated switchgrass and other biomass carbohydrates. BR1, 2 = Biological Replicate 1, 2, respectively.