Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis (original) (raw)
Figure 4
Weighted-Normalized Spectral Abundance Factor (Weighted-NSAF) of cellulosomal components in mass spectrometry analysis.
Weighted-NSAF data for 14N isotopologs across seven different samples is arranged in descending order of values for cellulose sample. For each cellulosomal sample, the average NSAF value of the various components was divided by the value for the scaffoldin protein CipA to obtain weighted-NSAF values. Heat plot representation shows weighted-NSAF distribution (Red, highest; Green, lowest) of 54 cellulosomal subunits. The proteins with top 10, and the next 10, highest weighted-NSAF values in each sample are highlighted in Yellow and Orange, respectively. Locus entries of newly detected cellulosome components are highlighted in Blue. Substrate legend: C = Cellulose, CX = Cellulose-Xylan, CP = Cellulose-Pectin, CPX = Cellulose-Pectin-Xylan, SWG = Pretreated Switchgrass, Cb = Cellobiose, ZT = Z-Trim®.