Adult-Generated Hippocampal Neurons Allow the Flexible Use of Spatially Precise Learning Strategies (original) (raw)
Figure 3
LTP in C57BL/6 mice with suppressed adult neurogenesis.
(A) In TMZ mice treatment prevented LTP in the dentate gyrus of hippocampal slices perfused with ACSF, but LTP was induced in control mice (ANOVA F(1,198) = 252.8, p<0.05). Previous studies showed that new, adult generated granule cells facilitate the weak LTP observed, while LTP induction in mature neurons is blocked by GABAergic inhibition under physiologic conditions (16, 17). (B) Using GABAA antagonist biculline a strong LTP was observed in both treated mice and controls. (C) Significant differences between TMZ mice and controls appeared only for ACSF-LTP. (D) A cumulative histogram for both ACSF (right) and bicuculline (left) perfused hippocampal slices. Dashed lines represent TMZ mice, solid lines controls. (E) LTP in hippocampal region CA1 was not affected by TMZ.